MISC

Basic information

Name OZAWA Hitoshi
Belonging department
Occupation name
researchmap researcher code 1000009178
researchmap agency Bukkyo University

Title

Heterogeneity in the pattern of distribution of the specific hormonal product and secretogranins within the secretory granules of rat prolactin cells.

Bibliography怀Type

Author

H Ozawa
R Picart
A Barret
C Tougard

OwnerRoles

 

Summary

We investigated the subcellular distribution of secretogranins I
II (Sg I
Sg II)
and prolactin (PRL) by double immunogold electron microscopy in GH3B6 rat pituitary tumor cells grown in different culture conditions and in normal PRL cells in adult male rat anterior pituitary. Co-localization of Sg I or Sg II with PRL was observed in most secretory granules in GH3B6 cells and normal PRL cells
except for some secretory granules containing only Sgs in GH3B6 cells and containing only PRL in normal PRL cells. In GH3B6 cells treated with thyrotropin-releasing hormone (TRH) for 2 hr
the newly formed small secretory granules within the Golgi zone contained preferentially immunoreactive PRL. Interestingly
when co-localized with PRL
Sgs (particularly Sg I) were observed at the periphery of the matrix of secretory granules in GH3B6 cells as well as in normal PRL cells
suggesting their possible interaction with the secretory granule membrane. The present study indicates a heterogeneous subcellular distribution of PRL
Sg I
and Sg II in individual secretory granules of GH3B6 cells and of normal PRL cells
pointing out the formation of different types of aggregates during the condensation of secretory products in these two PRL cell models.

Magazine(name)

Journal of Histochemistry & Cytochemistry

Publisher

SAGE Publications

Volume

42

Number Of Pages

8

StartingPage

1097

EndingPage

1107

Date of Issue

1994/08

Referee

 

Request

 

Language

 

Posting type

 

International Journal

 

International Collaboration

 

ISSN

 

eISSN

 

ISBN

 

DOI

10.1177/42.8.8027529

NAID

 

Cinii Books Id

 

PMID

 

PMCID

 

Format

Url

Download

 

J-GLOBAL ID

 

arXiv ID

 

ORCID Put Code

 

DBLP ID

 

Major Achivement