The general anesthetic sevoflurane affects the expression of clock gene mPer2 accompanying the change of NAD(+) level in the suprachiasmatic nucleus of mice

Yumiko Ohe
Norio Iijima
Kana Kadota
Atsuhiro Sakamoto
Hitoshi Ozawa

Sevoflurane is an anesthetic for the general anesthesia. In this study
 we showed that sevoflurane anesthesia affects the expression of mouse Per2 (mPer2)
 which is a clock gene in the brain which is considered the organ where the anesthetics act in. 64.5% of mPer2 circadian expression was repressed under anesthesia in the suprachiasmatic nucleus (SCN) of the brain. After recovering from the anesthesia
 the repressed mPer2 expression was restored to the same level as in non anesthesia-treated mice. This repression pattern was also observed in the subsequent phases of diurnal mPer2 expression. However
 obvious phase-shift in the mPer2 expression was not showed in this study. On the other hand
 the behavior analysis in this experiment exhibited that the phases in the circadian behavioral rhythm were shifted backwards. We also measured the NAD(+). level in the SCN
 which was a mediator regulating the mPer2 expression. Then
 significant increase of NAD(+) was detected under the anesthesia. These results indicate that the anesthesia induces the increase of NAD(+)
 and consequently leads to the repression of mPer2 expression and modifies the circadian expression pattern and diurnal behavioral rhythm of mice. Furthermore
 the modification of mPer2 expression by the anesthesia is considered to affect various gene expressions. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

NEUROSCIENCE LETTERS

ELSEVIER IRELAND LTD

490

3

10.1016/j.neulet.2010.12.059

https://doi.org/10.1016/j.neulet.2010.12.059
https://www.ncbi.nlm.nih.gov/pubmed/21195744
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