Serotonergic neurones in the dorsal raphe nucleus that project into the medial preoptic area contain oestrogen receptor beta

H Lu
H Ozawa
M Nishi
T Ito
M Kawata

Serotonin is involved in female sexual behaviour in which the medial preoptic area (MPA) has a pivotal role. The present study used immunohistochemistry
 in situ hybridization and retrograde transport analysis to investigate whether serotonin neurones in the dorsal raphe nucleus (DRN) of females projecting into the MPA contained oestrogen receptor alpha or beta. The projection of serotonin neurones from the DRN to the MPA was confirmed using the microinjection of Fluoro-Gold (FG)
 a fluorescent retrograde tracer
 into the MPA of ovariectomized (OVX-group) and OVX-rats treated with oestradiol benzoate (E2-group). A number of serotonin neurones in the DRN were labelled with FG
 indicating that these serotonin neurones in DRN project their terminals into the MPA. FG-labelled serotonin neurones expressed ER beta mRNA in the DRN
 and the number of the serotonin neurones containing ER beta mRNA between the OVX-group and the E2-treated group was not significantly different. Serotonin neurones in the DRN did not express ER alpha -immunoreactivity. Since previous findings showed that the density of serotonin-immunoreactive fibres and the concentration of serotonin within the MPA was significantly lower in the E2-group than the OVX-group
 our present observations suggested that the regulatory effects of E2 on the serotonergic neurone system in the MPA may be via ER beta within the serotonin-containing cells in the DRN of female rats.

JOURNAL OF NEUROENDOCRINOLOGY

BLACKWELL SCIENCE LTD

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