The effects of nerve growth factor on newborn rat dorsal root ganglion neurons in vitro: A scanning electron microscopic observation

YM Yang
K Yuri
T Matsuura
H Ozawa
M Kawata

The effects of nerve growth factor (NGF) on the three-dimensional structure of newborn rat dorsal root ganglion (DRG) neurons
 in vitro
 were examined by scanning electron microscopy. Throughout the culture in both NGF treated and control groups
 two types of structures of the surface were recognized
 smooth surfaced and rough surfaced neurons. Growth cones and varicosities of the processes were observed after 12 hours of culture in NGF-treated groups
 in contrast they were observed after 24 hours in control groups. The size and number of growth cone and varicosity was greater in NGF-treated groups than in control groups. NGF elongated the processes of DRG neurons after 24 hours of culture. NGF also significantly increased the number of surviving neurons after 48-72 hours of culture. Rough surfaced neurons were more sensitive than smooth surfaced neurons to NGF in enhancing survival. These results suggest that NGF may induce maturation of DRG neurons through elongation of its processes and the formation of growth cones and varicosities. Furthermore
 the perikaryal projections of DRG neurons may be related to neuron survival after NGF treatment.

JOURNAL OF BRAIN RESEARCH-JOURNAL FUR HIRNFORSCHUNG

AKADEMIE VERLAG GMBH

37

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