Imaging Analysis of Subcellular Correlation of Androgen Receptor and Estrogen Receptor α in Single Living Cells Using Green Fluorescent Protein Color Variants

Ikuo Ochiai
Ken-Ichi Matsuda
Mayumi Nishi
Hitoshi Ozawa
Mitsuhiro Kawata

Androgen and estrogen act not only in a sex-specific manner but also interactively and synergistically. In the present study
 to examine the possible interaction between androgen receptor (AR) and estrogen receptor-α (ERa)
 we investigated the subcellular dynamics of AR and ERα fused with green fluorescent protein color variants in single living cells using time-lapse microscopy and the technique of fluorescence recovery after photo-bleaching. AR and ERα showed punctate colocalization in the nucleus with estrogen
 but not androgen. N-terminal AR deletion mutant did not form a nuclear punctate pattern with either androgen or estrogen. In the presence of AR
 but not ERα
 N-terminal AR deletion mutant formed a punctate nuclear pattern with androgen. AR had different mobility depending on the ligand and the presence of ERα. On the other hand
 AR had little effect on the stability of ERα. ERα mutant that does not bind coactivators did not alter the mobility of AR. Taken together
 using an imaging technique
 we clarified that possible homo/hetero dimerization between AR and ERα could be attributed to androgen-estrogen interaction in living cells.

Molecular Endocrinology

18

1

10.1210/me.2002-0262

https://doi.org/10.1210/me.2002-0262
https://www.ncbi.nlm.nih.gov/pubmed/14563933