Expression of Glucocorticoid Receptor and Coactivators in Ependymal Cells of Male Rats

Kinuyo Iwata
Hitoshi Ozawa

Glucocorticoid receptor (GR) is a ligand-activated nuclear receptor which is widely distributed in the brain. Many types of neurons and glial cells are known to express GR
 but the expression of GR in ependymal cells has yet to be identified. The present study therefore was undertaken to determine whether ependymal cells express GR and coactivators of GR
 such as steroid receptor coactivator 1 (SRC-1) and p300. GR immunoreactivity was found in cells immunopositive to vimentin
 a marker of ependymal cells
 around the third ventricle (3V)
 the lateral ventricle (LV)
 the cerebral aqueduct and the fourth ventricle (4V)
 whereas the expression of GR in vimentin-immunoreactive (ir) cells was significantly reduced by adrenalectomy (ADX) in male rats. Vimentin-ir cells also expressed both SRC-1 and p300 at around 3V
 LV
 the cerebral aqueduct and 4V. ADX had no effect on the expression of SRC-1 or p300 in vimentin-ir cells. These results suggest that glucocorticoid may exert effects on ependymal cells through binding to GR followed by association with SRC-1 and p300 to maintain brain environment under stressful conditions.

ACTA HISTOCHEMICA ET CYTOCHEMICA

JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY

47

4

10.1267/ahc.14021

https://doi.org/10.1267/ahc.14021
https://www.ncbi.nlm.nih.gov/pubmed/25392570
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000342658800004&DestApp=WOS_CPL