Subunit profiling and functional characteristics of acetylcholine receptors in GT1-7 cells

Yuki Arai
Hirotaka Ishii
Makito Kobayashi
Hitoshi Ozawa

GnRH neurons form a final common pathway for the central regulation of reproduction. Although the involvement of acetylcholine in GnRH secretion has been reported
 direct effects of acetylcholine and expression profiles of acetylcholine receptors (AChRs) still remain to be studied. Using immortalized GnRH neurons (GT1-7 cells)
 we analyzed molecular expression and functionality of AChRs. Expression of the mRNAs were identified in the order alpha 7 > beta 2 = beta 1 ae alpha 4 ae alpha 5 = beta 4 = delta > alpha 3 for nicotinic acetylcholine receptor (nAChR) subunits and m4 > m2 for muscarinic acetylcholine receptor (mAChR) subtypes. Furthermore
 this study revealed that alpha 7 nAChRs contributed to Ca2+ influx and GnRH release and that m2 and m4 mAChRs inhibited forskolin-induced cAMP production and isobutylmethylxanthine-induced GnRH secretion. These findings demonstrate the molecular profiles of AChRs
 which directly contribute to GnRH secretion in GT1-7 cells
 and provide one possible regulatory action of acetylcholine in GnRH neurons.

JOURNAL OF PHYSIOLOGICAL SCIENCES

SPRINGER JAPAN KK

67

2

10.1007/s12576-016-0464-1

https://doi.org/10.1007/s12576-016-0464-1
https://www.ncbi.nlm.nih.gov/pubmed/27343174
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000394427900007&DestApp=WOS_CPL
http://orcid.org/0000-0003-2044-316X