Some anesthetics can affect gene expression. Previously
we reported that sevoflurane anesthesia drastically and reversibly repressed the expression of mouse Per2 (mPer2)
a core clock gene in the suprachiasmatic nucleus (SCN). In the current study
we examined the time-dependent effect of sevoflurane on mPer2 expression and its interactions with the circadian rest/activity rhythm of mice. During certain hours of the day
mice were anesthetized with 2.5% sevoflurane in 40% oxygen for 4 h. The expression level of mPer2 in the SCN was measured by in situ hybridization using a radiolabeled cRNA probe. Anesthesia during the morning hours showed the greatest repressive effect on mPer2 expression. Sevoflurane anesthesia repressed mPer2 expression during the conditions of light/dark and constant dark
and the light conditions modified the repression rate under anesthesia. Moreover
anesthesia in the morning also repressed mPer2 expression the following day. This dominant effect of anesthesia in the morning indicates that sevoflurane anesthesia affects the onset of mPer2 transcription. Behavior analysis revealed that the anesthetic treatment also induced a phase-delay in the rest/activity rhythm. However
no time-dependent effects of anesthesia on the circadian rest/activity rhythm were observed. Further investigation into the molecular events caused by anesthesia are required to explain atypical clinical signs observed in patients after surgical procedures
such as fatigue
sleep disorder
mood alteration and delirium. (c) 2012 Elsevier Ireland Ltd. All rights reserved.
