Pulsatile secretion of gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) decreases during aging. Kisspeptin (encoded by Kiss1) neurons in the arcuate nucleus coexpress neurokinin B (Tac3) and dynorphin (Pdyn) and are critical for regulating the GnRH/LH pulse. We therefore examined kisspeptin neurons by histochemistry and pulsatile LH release in rats aged 2-3 (Young)
12-13 (YoungMiddle)
19-22 (Late-Middle)
and 24-26 (Old) months. Total LH concentrations
sampled for 3 hours
decreased in both sexes with aging. In females
numbers of Tac3 and Pdyn neurons were significantly reduced in all aging rats
and numbers of Kiss1 neurons were significantly reduced in Late-Middle and Old rats. In males
numbers of all 3 neuron-types were significantly decreased in all aging rats. GnRH agonist induced LH release in all animals; however
the increased LH concentration in all aging rats was less than that in Young rats. These results suggest that expression of each gene in kisspeptin neurons may be controlled individually during aging
and that reduction of their expression or change in pituitary responsiveness may cause attenuated pulsatile LH secretion. (C) 2016 Elsevier Inc. All rights reserved.
